Journal: PLoS Pathogens

Article Title: Endothelial Cell Permeability during Hantavirus Infection Involves Factor XII-Dependent Increased Activation of the Kallikrein-Kinin System

doi: 10.1371/journal.ppat.1003470

Figure Lengend Snippet: Mock-, HTNV-, or ANDV-infected HUVEC were trypsinized, seeded onto ECIS chamberslides, and cultured until confluent. Media were removed and replaced with phenol red free EBM containing Zn 2+ . After an equilibration, 0.2 ml of phenol red free EBM containing Zn 2+ , FXII, PK, and HK (100 nM each) were added to cells (time zero) (black lines) (A). To measure inhibition of activation, some samples were treated with CTI (1 µM) (blue line), PKSI-527 (5 µM) (red line), or HOE 140 (1 µM) (green line) simultaneously with factors (B, C, & D). Real time measurements frequency measurements were taken throughout the assay.

Article Snippet: Arrays were then loaded into the ECIS 16-well array station and stabilized using ECIS software v1.2.92.0 (Applied Biophysics).

Techniques: Infection, Cell Culture, Inhibition, Activation Assay

Journal: Applied in Vitro Toxicology

Article Title: Inflammatory Response and Barrier Dysfunction by Different e-Cigarette Flavoring Chemicals Identified by Gas Chromatography–Mass Spectrometry in e-Liquids and e-Vapors on Human Lung Epithelial Cells and Fibroblasts

doi: 10.1089/aivt.2016.0030

Figure Lengend Snippet: Effects of nicotine and flavoring chemicals on electrical resistance in 16-HBE cells. 16-HBE cells were seeded in ECIS array/cultureware. Cells were grown for 2 days in complete medium with 10% FBS, then placed in medium containing low serum (1% FBS) and treated with nicotine or different flavoring chemicals (1 mM) and monitored for 16–24 hours. Resistance was measured at 4000 Hz using ECIS. (A) Representative data showing absolute resistance for control (no treatment), nicotine, and different flavoring chemicals. Black color arrow indicates the exact time of treatment. (B) Normalized resistance values for control, nicotine, and treatment with different flavoring chemicals (0 vs. 20 minutes post-treatment). ** p < 0.01 coumarin versus control; *** p < 0.001 diacetyl versus control. (C) Normalized resistance values for control and different flavoring chemicals 2 hours post-treatment. Statistical analysis of significance for normalized resistance values was compared between control vs. different flavoring chemicals at only 2-hour time point. Data are expressed as mean ± SEM ( n = 6–8/group), and significance determined using two-way ANOVA (Sidak's multiple comparisons test). * p < 0.05 cinnamaldehyde versus control; *** p < 0.001, acetoin and maltol versus control. ECIS, electric cell-surface impedance sensing; FBS, fetal bovine serum.

Article Snippet: Transepithelial resistance (TEER) data were collected in real time pre- and post-treatment (15 hours) using ECIS Zθ 16-well array station (Applied Biophysics, Troy, NY).

Techniques: Control